Isolation and characterization of an antarctic flavobacterium strain with agarase and alginate lyase activities
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Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
De Gruyter Open
Abstract
Several bacteria that are associated with macroalgae can use phycocolloids
as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta),
in King George Island, Antarctica, was screened and characterized for the ability
to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was
identified as a member of the genus Flavobacterium, closely related to Flavobacterium
faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic
due to its optimal temperature (17 ºC) and maximum temperature (20°C) of
growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C
to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose
(50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium
INACH002 is a promising Antarctic biotechnological resource; however, further research
is required to illustrate the structural and functional bases of the enzymatic performance
observed during the degradation of different substrates at different temperatures.
Description
Keywords
Ciencia, Marine bacterium, Molecular carachterization
Citation
Pol. Polar Res. 37 (3): 403–419, 2016