Browsing by Author "Lavin, Paris"
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Item Antibacterial activity of the antarctic bacterium Janthinobacterium sp. SMN 33.6 against multi-resistant Gram-negative bacteria(Pontificia Universidad Católica de Valparaíso, 2014) Asencio, Geraldine; Lavin, Paris; Alegria, Karen; Domínguez, Mariana; Bello, Helia; González-Rocha, Gerardo; González-Aravena, MarceloBackground: The increment of resistant strains to commonly used antibiotics in clinical practices places in evidence the urgent need to search for newcompounds with antibacterial activity. The adaptations that Antarctic microorganisms have developed, due to the extreme environment that they inhabit, promote themas a potential new source of active compounds for the control of microorganisms causing infections associated with health care. The aimof this studywas to evaluate the antibacterial activity of an ethanol extract of the Antarctic bacterium Janthinobacterium sp., strain SMN 33.6, against nosocomial multi-resistant Gram-negative bacteria. Results: Inhibitory activity against human Gram-negative bacterial pathogens, with concentrations that varied between 0.5 and 16 μg ml-1, was demonstrated. Conclusions: The ethanolic extract of Janthinobacterium sp. SMN 33.6 possesses antibacterial activity against a chromosomal AmpC beta-lactamase-producing strain of Serratia marcescens, an extended-spectrum beta-lactamase-producing Escherichia coli and also against carbapenemase-producing strains of Acinetobacter baumannii and Pseudomonas aeruginosa. This becomes a potential and interesting biotechnological tool for the control of bacteria with multi-resistance to commonly used antibiotics.Item Isolation and characterization of an antarctic flavobacterium strain with agarase and alginate lyase activities(De Gruyter Open, 2016) Lavin, Paris; Atala, Cristian; Gallardo-Cerda, Jorge; González-Aravena, Marcelo; De la Iglesia, Rodrigo; Oses, Rómulo; Torres-Díaz, Cristian; Trefault, Nicole; Molina-Montenegro, Marco A.; Laughinghouse IV, H. DailSeveral bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was identified as a member of the genus Flavobacterium, closely related to Flavobacterium faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic due to its optimal temperature (17 ºC) and maximum temperature (20°C) of growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose (50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium INACH002 is a promising Antarctic biotechnological resource; however, further research is required to illustrate the structural and functional bases of the enzymatic performance observed during the degradation of different substrates at different temperatures.